Friday, March 1, 2013

Family thank neighbours for help during Taunton garden fire (From ...

Family thank neighbours for help during Taunton garden fire

A FAMILY has thanked quick-thinking neighbours who put their lives on the line to help stop a raging fire reaching their house.

Vicki Moore, 28 and her husband Nick, 43, were watching television at their home in Cheddon Road, Taunton, on Friday (February 22) night when they heard two explosions.

Mr Moore investigated outside and when he opened the back door he was greeted with flames heading towards the house.

Mrs Moore said: ?My husband said ?get everybody out? as he tried to put the fire out.

?Three neighbours nearby helped him and it is just a massive thank you to them for ringing the fire service and looking after my husband.

?It was all a bit of a scare.?

Mrs Moore and her three children, Kaden, nine, Leon, eight, and Bethany, five, left through the front of the house to safety.

As Mr Moore tackled the flames at the back of the property he almost collapsed due to smoke inhalation but one of the neighbours arrived just in time to pull him away before taking over the hosepipe to tackle the flames.

Mr Moore was treated by ambulance crews and the family, who have lived at the house since 2008, say they are in the process of clearing up all the rubble the fire has left in their garden.

A Devon and Somerset Fire spokesman said they sent one fire engine and four firefighters to the scene and believe the fire started in a pile of rubbish at the back of the two-storey property.

Is community spirit still alive? Would you help your neighbours out? Let us know.

Source: http://www.somersetcountygazette.co.uk/news/taunton_news/10254326.Family_thank_neighbours_for_help_during_Taunton_garden_fire/?ref=rss

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Lipid nanoparticles are ideal for delivering genes and drugs, researchers show

Feb. 28, 2013 ? At the Faculty of Pharmacy of the Basque Public University (UPV/EHU) the Pharmacokinetics, Nanotechnology and Gene Therapy research team is using nanotechnology to develop new formulations that can be applied to drugs and gene therapy. Specifically, they are using nanoparticles to design systems for delivering genes and drugs; this helps to get the genes and drugs to the point of action so that they can produce the desired effect.

The research team has shown that lipid nanoparticles, which they have been working on for several years, are ideal for acting as vectors in gene therapy. Gene therapy is a highly promising alternative for diseases that so far have no effective treatment. It consists of delivering a nucleic acid, for example, a therapeutic gene, to modulate the expression of a protein that is found to be altered in a specific disease, thus reversing the biological disorder.

The main obstacle is that the genetic material cannot be formulated in conventional pharmaceutical ways, because it becomes degraded within the organism and cannot perform its function. To overcome this obstacle, viral vectors are normally used and they are able to deliver the therapeutic gene to the cells in which it has to act. However, as Dr Alicia Rodriguez explains, "viral vectors have a great drawback because they have a great potential to develop tumours. That is why there is a lot of interest in developing non-viral vectors, like vectors based on lipid nanoparticles. "

"In this respect," adds Dr Rodriguez, "we have for several years been working to develop formulations for treating degenerative retina diseases, diseases for which there is currently no effective curative or palliative treatment and which causes blindness in the patients who in many cases are very young people. "The research they have done has borne fruit already, and they have in fact managed to develop a vector capable of making a protein express itself in the eyes of rats after ocular delivery. The work has produced two patents and various papers published in top scientific journals, like Human Gene Therapy.

Aim: to improve drug absorption

Another application of lipid nanoparticles is to develop new formulations to deliver drugs that are not particularly soluble or which are difficult to absorb. Dr Rodriguez explained the problem with these drugs:"40% of the new pharmacologically active molecules are reckoned to be insoluble or not very soluble in water; that prevents many of these potentially active molecules from ever reaching the clinic because of the problems involved in developing a safe, effective formulation. "

The Faculty of Pharmacy's research team has shown that the strategy of encapsulating drugs of this type in lipid nanoparticles is effective:"They are spheres made of lipids and they have very small particles which encase the drug. That way, the absorption of the drug given orally can be increased," points out Dr Rodriguez.

Part of the research was done in collaboration with the research team led by Dr V?ronique Pr?at, of the Catholic University of Louvain in Belgium. There they studied the capacity of the nanoparticles to pass through the intestinal barrier and therefore increase the permeability of the drug. The results of this work have been published in the Journal of Controlled Release, a leading journal within the specialty.

Furthermore, while considerable advances have been made in both areas (vectors for gene therapy and improvement in insoluble drug absorption), the researchers in the Pharmacokinetics, Nanotechnology and Gene Therapy team are working in a third area linked to hepatitis C in which they also hope to achieve positive results.

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Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/health_medicine/genes/~3/bk8zSW-4U9I/130228080248.htm

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Thursday, February 28, 2013

Protests mark anniversary of Trayvon Martin's shooting death

SANFORD, Fla./NEW YORK (Reuters) - Demonstrators symbolically wearing hoodies gathered in New York, Florida and California on Tuesday to mark the anniversary of the shooting death of unarmed black teenager Trayvon Martin, reviving a national discussion on gun laws and racial profiling.

Actor Jamie Foxx joined Martin's parents and several hundred protesters for a candlelight vigil in New York City's Union Square, while a smaller crowd estimated at 110 to 125 met at a park in the Florida town where Martin died, vowing to continue to agitate for an end to racial discrimination.

"We want you (to) know we love you and we won't leave you," Foxx told Martin's mother, Sybrina Fulton, in New York.

Neighborhood watch volunteer George Zimmerman shot and killed Martin, 17, in the Orlando suburb of Sanford on February 26, 2012, and initially went free based on his claims of self defense. Then a national outcry forced the city's police chief to resign and the governor to appoint a special prosecutor.

Zimmerman now faces second-degree murder charges and a June trial. He has maintained his innocence, and supporters say he has been unfairly tainted as racist, noting the neighborhood had been hit by a wave of break-ins and that Zimmerman is of mixed race - his father is white and his mother Afro-Peruvian.

In Sanford, the case triggered deep emotions, and protesters staged a candlelight vigil and moment of silence at 7:15 p.m., the time Martin was killed, at Fort Mellon Park.

"There are no excuses for violence against our children. Let us take the tragedy of Trayvon's death and use it for good," said organizer Geri Hepburn, a white parent of a teenage son who became politically active as a result of the shooting.

The crowd was small compared to the thousands who filled the same park at the apex of public outrage of the killing last year, when the story dominated national news for weeks.

SYMBOLIC HOODIES

In New York, demonstrators recreated the "Million Hoodie March" of last year, when people wore hooded sweatshirts in the style worn by Martin the night of his death, when Zimmerman called police to report a suspicious looking person in his gated neighborhood and defied a police admonishment not to follow him.

The coast-to-coast series of events also saw a crowd gather in the Leimert Park section of Los Angeles. Participants carried lit candles and many of them also wore hoodies, said organizer Najee Ali, who spoke at the gathering in Los Angeles.

"Trayvon was everyone's son. He belonged to all of us," Ali said in a phone interview.

Martin was on his way home to the house of his father's girlfriend, and the hoodie became a symbol of what critics considered racial profiling.

"We are all Trayvon Martin," demonstrators chanted at Tuesday's vigil.

James Flood, 33, a black bartender and screenwriter, said he was constantly the victim of racial profiling and wanted better for his 11-year-old son.

"My skin color cannot change no matter how much money I make. I still get profiled," Flood said. "It has to stop."

Zimmerman, 29, who was released on bail, remained out of sight on the anniversary.

Thrust into the national spotlight, Martin's grieving parents, Fulton and father Tracy Martin, have become national advocates for stricter gun control laws and critics of Florida's Stand Your Ground law.

The law, passed in 2005, allows people to use lethal force in self defense if they are in fear of serious bodily harm. More than 20 states have since passed similar laws.

Police cited that law in initially declining to arrest Zimmerman, which sparked celebrity protests and popular demonstrations across the country, turning the case into an international story.

Zimmerman's attorney plans to invoke the Stand Your Ground law at an April 29 hearing at which a Florida judge could determine if the law applied to Zimmerman, possibly granting him immunity and averting a criminal trial.

(Additional reporting by Alex Dobuzinskis in Los Angeles; Writing by Daniel Trotta; Editing by Steve Orlofsky, Cynthia Osterman and Lisa Shumaker)

Source: http://news.yahoo.com/protests-mark-anniversary-trayvon-martin-death-012438318.html

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Loss Of Online Privacy Kills Free Speech - Business Insider

A few months back, an advertising executive argued that more online privacy would kill free speech.

Richard Frankel, president of the advertising company Rocket Fuel, titled the post ?How the Do Not Track Plan Will Ultimately Kill Free Speech.?

It?s understandable that advertisers are so resistant to the concept of Do Not Track ? it could change the way they access data, which would force them to innovate their business models and practices.?

Just because DNT could impact the advertising industry in the short-term, however, doesn?t warrant its intense broadcasting of fear, uncertainty and doubt (FUD), and at times, outright deception. Frankels?s piece was one in a string of ad exec attacks on privacy that claim that the Internet will be doomed if consumers get more of the privacy they want.

In the interest of transparency, let me explain my angle here. I?m an attorney, privacy advocate, and analyst at Abine, an online privacy company in Boston. We make simple tools that give people a choice over whether their personal info is collected, stored, and sold online.

Unlike the advertising companies, we don?t collect or sell any user data. We only get paid if users like our free products and choose to buy our premium ones. It?s an up-front relationship that?s clear to our customers.

And beyond my role at Abine, I care deeply about preserving the web as a place where we can say and explore interesting, sometimes controversial, things. As a fan of free speech and expression, I have a vested interest in keeping the Internet open and uncensored.

Make no mistake about it: we live in a state of surveillance. Hundreds of advertising and tracking companies follow everything we do online -- the articles we read, the videos we watch, the sites we always visit, the Facebook comments we make, and more.

They combine that online data with offline data like our voting record, employment history, and marriage licenses, and use it to build an extremely detailed profile. Companies like Facebook scan the contents of photos and private messages for Homeland Security ?risk words? like ?infection,? ?body scanner,? or ?hacker? and turn them over to law enforcement.

Even if you delete your embarrassing Facebook posts, companies like Social Intelligence sell the past 7 years of posts to hiring managers. The wireless companies you pay for mobile service turn over 1.3 million customer records to law enforcement each year, which include texts and your phone?s GPS location wherever it went.

The divide between public and private surveillance is virtually nonexistent, and advertising companies are part of this ecosystem.

When you?re constantly being watched, you necessarily lack privacy. And when you?re constantly being watched, you act differently. That seems like an obvious point to those of us who?ve belted out song lyrics alone in our cars but would be terrified to do the same on stage in front of thousands or who laugh at things with their friends that they?d never say in front of their bosses, but observation effects are also well-established in scientific research, law, and popular culture (Big Brother, anyone?).

Privacy scholar Neil M. Richards writes that ?surveillance inclines us to the mainstream and the boring?when we are watched while engaging in intellectual activities, broadly defined?thinking, reading, web-surfing, or private communications?we are deterred from engaging in thoughts or deeds that others might find deviant.?

Deviance just means difference: deviation from the norm, creativity, standing out from the crowd. If you knew you were being watched and that your activities may resurface down the road in a job interview, on a date, in a newspaper, you?d be less likely to go that political rally, that Alcoholics Anonymous meeting, that gay bar, or that protest, all things that you have a right to do that are valued parts of a free, democratic society.

With privacy, you have control over who gets to see what you?re doing and where you are. You get to be one way around your boss, another around your kids, another around your best friends since elementary school. You get to pick that divide.

But when you?re watched all the time and you never know how that information will be used or where it?ll turn up, you censor yourself everywhere. This is called the Panopticon Effect. All this openness that social networks insist we want by default, the pervasive data collection that advertisers argue is good for us: they make anyone who?s paying attention censor themselves.

A key part of free speech is anonymous speech. Anonymous speech is a constitutionally protected First Amendment right, and as the Supreme Court stated in McIntyre v. Ohio Elections Commission, ?an honorable tradition of advocacy and of dissent.?

Online advertising constantly fights against anonymity and pseudonymity, trying to learn everything about web users to unmask them and peel away layers of demographic info, interests, and behaviors. Many social networks like Facebook have real name policies, insisting that members use their full, legal names on their accounts or be banned.

Frankel says that ?With higher ad revenues comes more rich content that spurs vibrant discussion.? I disagree. You know what spurs vibrant discussion? People, especially people behind pseudonyms. Ever been on a message board? A Reddit thread?

The most ?vibrant? discussions happen when people feel sufficiently protected to be honest. Sure, some people hide behind pseudonyms to harass others, but the core of the First Amendment avoids censoring the positive, protected uses of speech just because certain bad actors may abuse it.

To say that advertising is the only thing driving creative content doesn?t give credit to humanity?s ingrained creativity. First, plenty of content providers get paid for their work directly, from recording artists to bestselling authors to journalists. 43% of Americans pay content creators?authors of magazine articles and books?for e-books, and there are 400,000 paid subscribers to the Wall Street Journal alone.

The majority of people blogging and posting on social media today create content out of a desire to express themselves that?s unrelated to money. Just talk to any one of the thousands of bloggers and podcasters, particularly the anonymous ones, who pay to host their own websites just to get their message out to the public.

Most of these people don?t blog or podcast for a living; they do it because they love it and because their message is important to them. There?s power?and satisfaction?in speaking one?s mind, and it exists independently from ad money.

Privacy isn?t a hindrance to free speech; it?s the driving force behind it. Privacy provides both the boundaries of and protection for the space in which we can be ourselves. Privacy nurtures self-expression, creativity, speaking your mind, associating with whomever you wish, and exploring your interests.?

These are the First Amendment?s protections:? freedom of speech, of association, and of assembly.? They?re so important for self-actualization and self-determination that our founders immortalized them in the Bill of Rights. Privacy isn?t about having something to hide; it?s about having something to live for.

Why the ad industry is wrong about Do Not Track

A few months back, an advertising executive argued that more online privacy would kill free speech. Richard Frankel, president of the advertising company Rocket Fuel, titled the post ?How the Do Not Track Plan Will Ultimately Kill Free Speech.?

It?s understandable that advertisers are so resistant to the concept of Do Not Track ? it could change the way they access data, which would force them to innovate their business models and practices.? Just because DNT could impact the advertising industry in the short-term, however, doesn?t warrant its intense broadcasting of fear, uncertainty and doubt (FUD), and at times, outright deception.

Frankels?s piece was one in an ongoing string of ad exec attacks on privacy that claim that the Internet will be doomed if consumers get more of the privacy they want.

In the interest of transparency, let me explain my angle here. I?m an attorney, privacy advocate, and analyst at Abine, an online privacy company in Boston. We make simple tools that give people a choice over whether their personal info is collected, stored, and sold online. Unlike the advertising companies, we don?t collect or sell any user data.

We only get paid if users like our free products and choose to buy our premium ones. It?s an up-front relationship that?s clear to our customers. And beyond my role at Abine, I care deeply about preserving the web as a place where we can say and explore interesting, sometimes controversial, things.

As a fan of free speech and expression, I have a vested interest in keeping the Internet open and uncensored.

Make no mistake about it: we live in a state of surveillance. Hundreds of advertising and tracking companies follow everything we do online -- the articles we read, the videos we watch, the sites we always visit, the Facebook comments we make, and more.

They combine that online data with offline data like our voting record, employment history, and marriage licenses, and use it to build an extremely detailed profile. The divide between public and private surveillance is virtually nonexistent, and advertising companies are part of this ecosystem.

In this post, I?ll address Frankel?s argument - and the other advertisers using similar arguments - which goes something like this: 1) behavioral (or tracking-based) advertising is how online advertisers make money; 2) without advertising, content providers like online newspapers won?t be able to survive; 3) with paid subscriptions; only rich people who can afford content will get it, creating class segmentation.

In brief, here?s why they?re wrong:

1) the majority of online advertising revenue comes from contextual ads, which don?t pose any personal privacy risks;
2), the online advertising industry?s move to real-time bidding platforms are actually hurting content providers far more than a shift from behavioral advertising to contextual advertising ever could; and
3), beyond this being a reductio ad absurdum argument (?everything will be locked behind a paywall if we stop tracking!?), this segmentation already exists through the filter bubble that the advertising industry and its tracking has caused.

Realistically, online privacy isn?t going to hurt anything but these advertisers? antiquated business models, which will have to adapt to respect the privacy of their target audiences.

Let?s first examine the fallacy that online tracking doesn?t use personal information. Frankel argues that ?Targeted, relevant ads don?t harm consumers?personal information is not necessary, or collected, to produce them.?

This statement is less than fully honest when we look at Rocket Fuel?s own website, which boasts that it can ?zero in on? people based on ?age, gender, profession, ethnicity, and relationship status,? among many other personal characteristics. They have more than 20,000 audience segments, including soccer moms and caregivers:

The company goes on to say that they can find ?users engaged in highly targeted activities that define their interests and personalities? by going ?beyond behavioral, contextual, or geo-targeting by combining intelligent demographic, lifestyle, purchase-intent, and social data with our own suite of targeting algorithms, blended analytics, and expert analysis.?

And they?re not just using your online activity here:? ?Online data has evolved from simply providing insights like browsing activity, shopping cart info, sign ups, etc. into an incredible selection of more than 20,000 unique audience segments. Advertisers can even leverage offline purchase and consumption data for their online campaigns.?

Obviously, significant amounts of personal information are being collected. And the traditional advertiser counter-argument that ?it?s not personally identifiable; it?s only aggregate info? has been debunked time and time again by researchers like Stanford?s Arvind Narayanan and Jonathan Mayer (also see privacy professor Paul Ohm?s excellent summary of the failure of anonymization) and publications like the Wall Street Journal, which found in a December 2012 study that nearly 25% of the web?s 70 most popular sites shared personal data, like name and email address, with third-party companies.

Ad companies like Mixpanel come right out and say ?Now you can tie any kind of data to your users to see exactly who they are and what they have done.? The myth that de-identified data is private is even weaker now with the rise of ?data enhancement? that matches online info with offline data sets.

The only reason these ads can be personalized is because of the personal information, the data collection, that powers them. That?s the real harm here, and that?s always been the harm, despite advertisers trying to shift the focus to targeted ads. No one cares about targeted ads: at worst, they?re annoying or creepy. Let?s just drop it.

The thing people don?t like is having their personal info harvested, mined, sold, and used in ways they can?t even imagine: for determining their credit limits or creditworthiness, playing into whether they get a job, showing them different prices than other people see for the same online shopping items, or influencing their insurance rates.

Even Frankel seems to doubt himself: he says that ?consumers have become more open to [ads],? and then contradicts himself 6 paragraphs later when he says ?Everyone claims to hate online advertising.? His second statement is more accurate: an October 2012 UC Berkeley study found that most people--69%--never or hardly ever find ads useful, and 85% never or hardly ever click them; and a 2012 Pew phone survey of 2,253 participants found that 68% of them were ?not okay with targeted advertising because [they] don?t like having [their] online behavior tracked and analyzed.?

Microsoft surveyed their users about privacy in 2012 and found that 83% of Americans, 84% of Germans, and 81% of UK residents think that ?tracking of personal information is out of hand and consumers need easier ways to block it.? I won?t keep going; you get the picture. The advertising industry keeps preaching about how great data collection is for all of us. If the benefits are so clear, then why not let consumers choose to enable it? Overwhelmingly, the opposite is happening.

Let?s fact-check some of Frankel?s, and the advertising industry in general?s, more unsubstantiated statements:

Online ads have become ?less invasive?

If by ?invasive? he means ?in your face? like pop-ups, then he?s right. But just because data collection is invisible doesn?t mean it?s somehow safer or less intrusive. Advertisers employ a massive array of secret tracking techniques, collecting far more personal information than ever before in history, and they haven?t had the best rap while doing it: remember KISSmetrics creating undeletable cookies?

Google circumventing privacy controls in Safari and getting a $22.5 million FTC fine? Ad network Chitika?s opt-out that only lasted 10 days in contrast to any reasonable consumer?s expectations? Facebook tracking logged-out users anywhere there?s a Like button on the web?

Online tracking is only getting worse: UC Berkeley?s Web Privacy Census, powered by Abine?s privacy software, found an alarming increase in tracking on the top US websites, showing that online tracking will double in 2.5 years if present trends continue.

?The alternative to an ad-supported Internet is a pay-for-play world supported by subscriptions or private ownership. Consumers may think they want an ad-free Internet, but do we really want to pay subscription fees to access all the sites we currently visit for free??

Frankel wrongly equates ?behavioral advertising? with ?all advertising? and offers a false choice between an ad-supported Internet and an all-paid subscription Internet. He neglects to mention that the vast majority of advertising revenue comes from contextual ads, ads that relate to the content the viewer sees and not the personal characteristics of the viewer.

Contextual ads don?t present a privacy problem, and they make up the majority of online advertisements. The reality is that privacy controls will not have a negative impact on the economics of the Internet (at least not past this short-term period of transition), as I?ve argued elsewhere.

And it?s not as though advertisers are truthfully concerned about publishers? bottom lines. Significant evidence suggests the opposite: although online advertising is getting cheaper for advertisers, it?s getting more costly for publishers.

In the past, publishers had the power when pricing ads. You wanted a full-page New York Times ad? There?s a set price for that. With the recent advent of real-time online bidding platforms, advertisers hurt content providers by competing more effectively and cutting down the content provider?s take and involvement.

And at bottom, there?s plenty of research showing that plenty of people will pay small price increases for more privacy. A few dollars is all that?s needed to make the difference, so we?re not talking about a prohibitive expense. Offer people a choice: those who don?t mind surrendering their privacy can keep the status quo, but people who want to avoid it can throw in a few bucks to do so.

The Do Not Track option could serve as the dividing line to signal a consumer?s choice about privacy. Of course, it would need to live up to consumer expectations and actually stop tracking on websites, which notable websites like Twitter have done.

Do Not Track would ?reduc[e] the effectiveness of advertising?

To the contrary. Do Not Track has great potential to increase the effectiveness of advertising. Why? Because Do Not Track lets people tell advertisers not to use certain personalized ads that they find ineffective, usually because they find them creepy or annoying.

Do Not Track is a clear communication from web users to advertisers about which ads those users prefer; which ads work better for them. If ads annoy people and make them build negative associates with the things advertised, they?re not effective advertising.

Even with Do Not Track, advertisers still get to collect and sell user data for advertising: they just can?t show personalized online ads. According to the definition of Do Not Track offered by advertisers (which is at odds with what real people want), people who don?t want to be tracked will still see ads, but not personalized ones. Meanwhile, all other web users will keep seeing behaviorally targeted ads.

Across the board?regardless of whether a consumer clicked Do Not Track?advertisers will still collect all consumers? personal data exactly as before. Again, their definition, not ours.? This definition means that consumers get to voice which type of ads they prefer?personalized or not?and advertisers can still collect and sell everyone?s info. It?s a win-win for advertisers, far from the end of the Internet economy and a blow to advertising effectiveness.

?If independent publishers lose a large percentage of their ad revenues because Do Not Track or other initiatives restrict the free flow of information on the web, they?ll have less money to fund unbiased, journalistic content creation?

We already have a restricted flow of free information on the web, thanks to advertising. Ever heard of a filter bubble? It?s ?a situation in which a website algorithm selectively guesses what information a user would like to see based on information about the user (such as location, past click behavior and search history) and, as a result, users become separated from information that disagrees with their viewpoints, effectively isolating them in their own cultural or ideological bubbles.?

In other words, the more that advertisers or websites (or both, in the case of sites like Google and Facebook) know about you, the more they envelop you with targeted content. You don?t see the same Google search results as everyone else, or the same Amazon home page. Even news sites are targeted to show you articles they think you?ll like. Personal data collection fuels filter bubbles.

Frankel says that greater privacy will ?muffle the voices of many consumers who can?t or won?t pay to express their opinions.? You know what muffles voices? Surveillance, tracking, and the threat that what you say today will be used against you in the future.

That?s why anonymous speech is constitutionally protected and why many people choose to mask themselves when they make political comments. Online advertising works extremely hard to unmask those people, identify them, sell their data, and barrage them with ads. And that?s not what people want.

Source: http://www.businessinsider.com/loss-of-online-privacy-kills-free-speech-2013-2

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New cars increasingly out of reach for many

Looking to buy a new car, truck or crossover? You may find it more difficult to stretch the household budget than you expected, according to a new study that finds median-income families in only one major U.S. city actually can afford the typical new vehicle.

The typical new vehicle is now more expensive than ever, averaging $30,550 in 2012, according to TrueCar.com data, and heading up again as makers curb the incentives that helped make their products more affordable during the recession when they were desperate for sales.

According to the 2013 Car Affordability Study by Interest.com, only in Washington, D.C., could the typical household swing the payments, the median income there running $86,680 a year. At the other extreme, Tampa was at the bottom of the 25 large cities included in the study, with a median household income of $43,832.

The study looked at a variety of household expenses, such as food and housing, and when it comes to purchasing a new vehicle, it considered more than just the basic purchase price, down payment and monthly note, factoring in such essentials as taxes and insurance.

Bottom line? A buyer in the capital can purchase a car with a sticker price of $31,940, slightly more than the new vehicle average for the 2013 model year and about what it would cost for a mid-range Ford Fusion sedan or a stripped-down BMW X1 crossover. The buyer in Tampa? They?ll just barely cover the cost of a basic Kia Rio, with $14,516 to spend.

More from The Detroit Bureau: Hyundai settles lawsuit for false mileage claims

?If you live in New York City or San Francisco, you?re probably going to have to pay a lot for housing, but you don?t have to pay a lot for a car,? said Mike Sante, the managing editor of Interest.com, a financial decision-making website.

Affordability has been a matter of growing concern for the auto industry in recent years as prices have continued to move upward. Even the most basic of today?s cars are generally loaded with features that were once found on high-line models a few decades back ? if they were available at all ? such as air conditioning, power windows, airbags and electronic stability control, as well as digital infotainment systems. They also have to meet ever tougher federal safety, emissions and mileage standards that have added thousands to the typical price tag.

?The average compact car of today has the features of a midsize model somebody might be trading in ? but it may be just as expensive,? said David Sargent, director of automotive operations for J.D. Power and Associates.

That is one reason why many buyers have been downsizing in recent years, said Bill Fay, general manager of Toyota, though he added that ?there is still a lot of affordability in the marketplace.?

Perhaps, but industry planners have come to recognize that they are targeting a much smaller segment of the American public than in decades past. That?s one reason why most manufacturers are offering more downsized models.

More from The Detroit Bureau: Consumer Reports names its 'Top Picks'

They also are working with their dealers to offer certified pre-owned programs where buyers can stretch their budget by purchasing a two- or three-year-old vehicle that has gone through an extensive inspection and, if necessary, repairs and replacements. Such vehicles may cost slightly more than a conventional used model but usually include a like-new warranty.

While the typical new vehicle will likely nudge up this year, Interest.com editor Sante stressed that car costs are one of the most controllable parts of a household?s budget. ?You?re better off driving something more affordable and saving or investing the difference.?

If the typical new car costs $30,550, with an average monthly payment of $550, the five cities most able to meet ? or come close ? are:

1) Washington, D.C.
Average Household Income: $86,680
Affordable Purchase Price: $31,940
Maximum monthly payment: $628

2) San Francisco
Average Household Income: $71,975
Affordable Purchase Price: $26,786
Maximum monthly payment: $537

3) Boston
Average Household Income: $69.455
Affordable Purchase Price: $26,025
Maximum monthly payment: $507

4) Baltimore
Average Household Income: $65,463
Affordable Purchase Price: $24,079
Maximum monthly payment: $468

5) Minneapolis
Average Household Income: $63,352
Affordable Purchase Price: $24,042
Maximum monthly payment: $470

At the other end of the scale, those five cities least able to handle a car payment are:

21) Phoenix
Average Household Income: $50,058
Affordable Purchase Price: $17,243
Maximum monthly payment: $348

22) San Antonio
Average Household Income: $48,699
Affordable Purchase Price: $17,137
Maximum monthly payment: $334

23) Detroit
Average Household Income: $48,968
Affordable Purchase Price: $17,093
Maximum monthly payment: $332

24) Miami
Average Household Income: $45,407
Affordable Purchase Price: $15,188
Maximum monthly payment: $295

25) Tampa
Average Household Income: $43,832
Affordable Purchase Price: $14,516
Maximum monthly payment: $282

More from The Detroit Bureau

Copyright ? 2009-2013, The Detroit Bureau

Source: http://www.nbcnews.com/business/new-cars-increasingly-out-reach-many-americans-1C8573730

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Long-term use of medication does not improve symptoms for heart failure patients

Feb. 26, 2013 ? Among patients with heart failure with preserved ejection fraction, long-term treatment with the medication spironolactone improved left ventricular diastolic function but did not affect maximal exercise capacity, patient symptoms, or quality of life, according to a study appearing in the February 27 issue of JAMA.

"Heart failure (HF) with preserved ejection fraction [EF; the percentage of blood that is pumped out of a filled ventricle as a result of a heartbeat is 50 percent or greater] accounts for more than 50 percent of the total HF population," according to background information in the article. There is not an established therapy for this condition, and aldosterone (a hormone) stimulation may contribute to its progression.

Frank Edelmann, M.D., of the University of Gottingen, Germany, and colleagues conducted a study to examine the long-term effects of spironolactone, an aldosterone receptor blocker, on diastolic function and exercise capacity in patients with HF with preserved EF. The Aldo-DHF trial, a randomized, placebo-controlled trial, was conducted between March 2007 and April 2012 at 10 sites in Germany and Austria. The study included 422 patients (average age, 67 years) with chronic New York Heart Association class II or III heart failure, preserved left ventricular ejection fraction of 50 percent or greater, and evidence of diastolic dysfunction. Patients were randomly assigned to receive 25 mg of spironolactone once daily (n = 213) or matching placebo (n = 209) with 12 months of follow-up. The primary outcomes measured were changes in diastolic function (E/e') on echocardiography and maximal exercise capacity (peak VO2) on cardiopulmonary exercise testing.

The researchers found that spironolactone improved some measures (left ventricular end-diastolic filling, left ventricular remodeling, and neurohumoral activation). Maximal exercise capacity did not significantly change with spironolactone vs. placebo, and spironolactone did not improve heart failure symptoms or quality of life and slightly reduced 6-minute walking distance. "Spironolactone also modestly increased serum potassium levels and decreased estimated glomerular filtration rate without affecting hospitalizations."

The authors conclude that the "lack of accepted minimal clinically important differences in E/e' or peak VO2 in HF with preserved EF warrants additional prospective, randomized, adequately powered studies to further evaluate the effect of improving diastolic function on symptomatic, functional, and clinical end points."

Editorial: Defining Diastolic Heart Failure and Identifying Effective Therapies

"Ultimately, Aldo-DHF trial provides valuable new information but is not particularly reassuring in terms of either the efficacy or safety of mineralocorticoid antagonists (MRAs) for patients with heart failure with preserved ejection fraction (HFpEF),"writes John G. F. Cleland, M.D., Ph.D., F.R.C.P., and Pierpaolo Pellicori, M.D., of the University of Hull, Kingston-upon-Hull, England, in an accompanying editorial.

"In the meantime, MRAs appear useful for managing congestion and preventing diuretic-induced hypokalemia [abnormally low level of potassium in the circulating blood] with the attendant risk of sudden arrhythmic death. It is likely that these benefits are independent of cardiac phenotype but might be more prominent in those with impaired aldosterone degradation due to hepatic congestion. Whether MRAs exert important benefits for patients with HFpEF through other mechanisms such as reducing fibrosis, inflammation, and adrenergic activity may take longer to unravel."

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The above story is reprinted from materials provided by American Medical Association (AMA).

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Journal Reference:

  1. Frank Edelmann et al. Effect of Spironolactone on Diastolic Function and Exercise Capacity in Patients With Heart Failure With Preserved Ejection FractionThe Aldo-DHF Randomized Controlled Trial. JAMA, 2013 DOI: 10.1001/jama.2013.905

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Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/most_popular/~3/CPq_J5Fjdoo/130226162725.htm

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New study shows viruses can have immune systems

New study shows viruses can have immune systems [ Back to EurekAlert! ] Public release date: 27-Feb-2013
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Contact: Siobhan Gallagher
siobhan.gallagher@tufts.edu
617-636-6586
Tufts University, Health Sciences Campus

A pirate phage commandeers the immune system of bacteria

BOSTON (February 27, 2013, embargoed until 1 p.m. US ET) A study published today in the journal Nature reports that a viral predator of the cholera bacteria has stolen the functional immune system of bacteria and is using it against its bacterial host. The study provides the first evidence that this type of virus, the bacteriophage ("phage" for short), can acquire a wholly functional and adaptive immune system.

The phage used the stolen immune system to disable and thus overcome the cholera bacteria's defense system against phages. Therefore, the phage can kill the cholera bacteria and multiply to produce more phage offspring, which can then kill more cholera bacteria. The study has dramatic implications for phage therapy, which is the use of phages to treat bacterial diseases. Developing phage therapy is particularly important because some bacteria, called superbugs, are resistant to most or all current antibiotics.

Until now, scientists thought phages existed only as primitive particles of DNA or RNA and therefore lacked the sophistication of an adaptive immune system, which is a system that can respond rapidly to a nearly infinite variety of new challenges. Phages are viruses that prey exclusively on bacteria and each phage is parasitically mated to a specific type of bacteria. This study focused on a phage that attacks Vibrio cholerae, the bacterium responsible for cholera epidemics in humans.

Howard Hughes Medical Institute investigator Andrew Camilli, Ph.D., of Tufts University School of Medicine led the research team responsible for the surprising discovery.

First author Kimberley D. Seed, Ph.D., a postdoctoral fellow in Camilli's lab, was analyzing DNA sequences of phages taken from stool samples from patients with cholera in Bangladesh when she identified genes for a functional immune system previously found only in some bacteria (and most Archaea, a separate domain of single-celled microorganisms).

To verify the findings, the researchers used phage lacking the adaptive immune system to infect a new strain of cholera bacteria that is naturally resistant to the phage. The phage were unable to adapt to and kill the cholera strain. They next infected the same strain of cholera bacteria with phage harboring the immune system, and observed that the phage rapidly adapted and thus gained the ability to kill the cholera bacteria. This work demonstrates that the immune system harbored by the phage is fully functional and adaptive.

"Virtually all bacteria can be infected by phages. About half of the world's known bacteria have this adaptive immune system, called CRISPR/Cas, which is used primarily to provide immunity against phages. Although this immune system was commandeered by the phage, its origin remains unknown because the cholera bacterium itself currently lacks this system. What is really remarkable is that the immune system is being used by the phage to adapt to and overcome the defense systems of the cholera bacteria. Finding a CRISPR/Cas system in a phage shows that there is gene flow between the phage and bacteria even for something as large and complex as the genes for an adaptive immune system," said Seed.

"The study lends credence to the controversial idea that viruses are living creatures, and bolsters the possibility of using phage therapy to treat bacterial infections, especially those that are resistant to antibiotic treatment," said Camilli, professor of Molecular Biology & Microbiology at Tufts University School of Medicine and member of the Molecular Microbiology program faculty at the Sackler School of Graduate Biomedical Sciences at Tufts University.

Camilli's previous research established that phages are highly prevalent in stool samples from patients with cholera, implying that phage therapy is happening naturally and could be made more effective. In addition, a study published by Camilli in 2008 determined that phage therapy works in a mouse model of cholera intestinal infection.

The team is currently working on a study to understand precisely how the phage immune system disables the defense systems of the cholera bacteria. This new knowledge will be important for understanding whether the phage's immune system could overcome newly acquired or evolved phage defense systems of the cholera bacteria, and thus has implications for designing an effective and stable phage therapy to combat cholera.

###

Additional authors are David W. Lazinski, Ph.D., senior research associate in the Camilli lab at Tufts University School of Medicine, and Stephen B. Calderwood, M.D., Morton N. Swartz, M.D. academy professor of medicine at Harvard Medical School, and chief, division of infectious disease and vice-chair, department of medicine at Massachusetts General Hospital.

Research reported in this publication was supported by the National Institute of Allergies and Infectious Diseases of the National Institutes of Health under award numbers R01AI55058, R01AI045746, and R01AI058935.

Seed, K.D., Lazinski, D.W., Calderwood, S.B., and Camilli, A. (2013). A bacteriophage encodes its own CRISPR/Cas adaptive response to evade host innate immunity. Nature, vol 494, issue 7438, pp 489 DOI: 10.1038/nature11927

About Tufts University School of Medicine and the Sackler School of Graduate Biomedical Sciences

Tufts University School of Medicine and the Sackler School of Graduate Biomedical Sciences at Tufts University are international leaders in innovative medical education and advanced research. The School of Medicine and the Sackler School are renowned for excellence in education in general medicine, biomedical sciences, special combined degree programs in business, health management, public health, bioengineering and international relations, as well as basic and clinical research at the cellular and molecular level. Ranked among the top in the nation, the School of Medicine is affiliated with six major teaching hospitals and more than 30 health care facilities. Tufts University School of Medicine and the Sackler School undertake research that is consistently rated among the highest in the nation for its effect on the advancement of medical science.

If you are a member of the media interested in learning more about this topic, or speaking with a faculty member at the Tufts University School of Medicine or another Tufts health sciences researcher, please contact Siobhan Gallagher at 617-636-6586 or siobhan.gallagher@tufts.edu.



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New study shows viruses can have immune systems [ Back to EurekAlert! ] Public release date: 27-Feb-2013
[ | E-mail | Share Share ]

Contact: Siobhan Gallagher
siobhan.gallagher@tufts.edu
617-636-6586
Tufts University, Health Sciences Campus

A pirate phage commandeers the immune system of bacteria

BOSTON (February 27, 2013, embargoed until 1 p.m. US ET) A study published today in the journal Nature reports that a viral predator of the cholera bacteria has stolen the functional immune system of bacteria and is using it against its bacterial host. The study provides the first evidence that this type of virus, the bacteriophage ("phage" for short), can acquire a wholly functional and adaptive immune system.

The phage used the stolen immune system to disable and thus overcome the cholera bacteria's defense system against phages. Therefore, the phage can kill the cholera bacteria and multiply to produce more phage offspring, which can then kill more cholera bacteria. The study has dramatic implications for phage therapy, which is the use of phages to treat bacterial diseases. Developing phage therapy is particularly important because some bacteria, called superbugs, are resistant to most or all current antibiotics.

Until now, scientists thought phages existed only as primitive particles of DNA or RNA and therefore lacked the sophistication of an adaptive immune system, which is a system that can respond rapidly to a nearly infinite variety of new challenges. Phages are viruses that prey exclusively on bacteria and each phage is parasitically mated to a specific type of bacteria. This study focused on a phage that attacks Vibrio cholerae, the bacterium responsible for cholera epidemics in humans.

Howard Hughes Medical Institute investigator Andrew Camilli, Ph.D., of Tufts University School of Medicine led the research team responsible for the surprising discovery.

First author Kimberley D. Seed, Ph.D., a postdoctoral fellow in Camilli's lab, was analyzing DNA sequences of phages taken from stool samples from patients with cholera in Bangladesh when she identified genes for a functional immune system previously found only in some bacteria (and most Archaea, a separate domain of single-celled microorganisms).

To verify the findings, the researchers used phage lacking the adaptive immune system to infect a new strain of cholera bacteria that is naturally resistant to the phage. The phage were unable to adapt to and kill the cholera strain. They next infected the same strain of cholera bacteria with phage harboring the immune system, and observed that the phage rapidly adapted and thus gained the ability to kill the cholera bacteria. This work demonstrates that the immune system harbored by the phage is fully functional and adaptive.

"Virtually all bacteria can be infected by phages. About half of the world's known bacteria have this adaptive immune system, called CRISPR/Cas, which is used primarily to provide immunity against phages. Although this immune system was commandeered by the phage, its origin remains unknown because the cholera bacterium itself currently lacks this system. What is really remarkable is that the immune system is being used by the phage to adapt to and overcome the defense systems of the cholera bacteria. Finding a CRISPR/Cas system in a phage shows that there is gene flow between the phage and bacteria even for something as large and complex as the genes for an adaptive immune system," said Seed.

"The study lends credence to the controversial idea that viruses are living creatures, and bolsters the possibility of using phage therapy to treat bacterial infections, especially those that are resistant to antibiotic treatment," said Camilli, professor of Molecular Biology & Microbiology at Tufts University School of Medicine and member of the Molecular Microbiology program faculty at the Sackler School of Graduate Biomedical Sciences at Tufts University.

Camilli's previous research established that phages are highly prevalent in stool samples from patients with cholera, implying that phage therapy is happening naturally and could be made more effective. In addition, a study published by Camilli in 2008 determined that phage therapy works in a mouse model of cholera intestinal infection.

The team is currently working on a study to understand precisely how the phage immune system disables the defense systems of the cholera bacteria. This new knowledge will be important for understanding whether the phage's immune system could overcome newly acquired or evolved phage defense systems of the cholera bacteria, and thus has implications for designing an effective and stable phage therapy to combat cholera.

###

Additional authors are David W. Lazinski, Ph.D., senior research associate in the Camilli lab at Tufts University School of Medicine, and Stephen B. Calderwood, M.D., Morton N. Swartz, M.D. academy professor of medicine at Harvard Medical School, and chief, division of infectious disease and vice-chair, department of medicine at Massachusetts General Hospital.

Research reported in this publication was supported by the National Institute of Allergies and Infectious Diseases of the National Institutes of Health under award numbers R01AI55058, R01AI045746, and R01AI058935.

Seed, K.D., Lazinski, D.W., Calderwood, S.B., and Camilli, A. (2013). A bacteriophage encodes its own CRISPR/Cas adaptive response to evade host innate immunity. Nature, vol 494, issue 7438, pp 489 DOI: 10.1038/nature11927

About Tufts University School of Medicine and the Sackler School of Graduate Biomedical Sciences

Tufts University School of Medicine and the Sackler School of Graduate Biomedical Sciences at Tufts University are international leaders in innovative medical education and advanced research. The School of Medicine and the Sackler School are renowned for excellence in education in general medicine, biomedical sciences, special combined degree programs in business, health management, public health, bioengineering and international relations, as well as basic and clinical research at the cellular and molecular level. Ranked among the top in the nation, the School of Medicine is affiliated with six major teaching hospitals and more than 30 health care facilities. Tufts University School of Medicine and the Sackler School undertake research that is consistently rated among the highest in the nation for its effect on the advancement of medical science.

If you are a member of the media interested in learning more about this topic, or speaking with a faculty member at the Tufts University School of Medicine or another Tufts health sciences researcher, please contact Siobhan Gallagher at 617-636-6586 or siobhan.gallagher@tufts.edu.



[ Back to EurekAlert! ] [ | E-mail | Share Share ]

?


AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert! system.


Source: http://www.eurekalert.org/pub_releases/2013-02/tuhs-nss022513.php

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